In this issue

Antibacterial Potential of Gracilaria edulis Extracts Against Pathogenic Bacteria: Input to Organic Aquaculture
Identification of Plankton in the Stomach of Milkfish (Chanos chanos) Seeds Obtained from Natural Waters
First Record of Hermaphroditism in Green Mussels (Perna viridis) in the Philippines
The Post-Harvest Handling Practices of Glass Eel in Aparri, Cagayan: Operations, Challenges and Recommendations
Dietary Vitamin C and Lipid Feed Supplementations Influence the Expression of Immune-Related Genes in Litopenaeus vannamei (Boone 1931) Subjected to Thermal Stress
Gender Participation in the Capture and Marketing Sectors of the Glass Eel Fisheries in Aparri, Cagayan, Philippines
Status of Nematopalaemon tenuipes, Spider Prawn (Family: Palaemonidae) Stocks Caught in Aparri, Cagayan: A case of Gentleman’s Agreement Management Strategy
Environmental Change and Trends in Brackishwater Pond Milkfish Chanos chanos Production in the Philippines Over Six Decades: Insights to Possible Resiliency
Coral Reef Habitat and Associated Reef Fishes in the Kalayaan Island Group, West Philippine Sea
Catches and the Production of the Sea Cucumber Fishery of Mindanao, Philippines
Ichthyofauna of the Rasian and Asahan Rivers in the South Coast of Aceh Province, Indonesia
Determinants of Food Insecurity among Municipal Fishing Households during the COVID-19 Pandemic under Alert Level 1 in Kawit, Cavite, Philippines
Cell Viability and Coelomocyte Characterization of Crown-of-Thorns Starfish (Acanthaster planci L.) Collected from Southern Leyte, Philippines
Farming Systems of Eucheumatoid Seaweeds in Western Visayas, Philippines
Climate and Non-climate Related Hazards in Small Pelagic Fisheries and Milkfish Aquaculture: Expert Opinion Survey in the Philippines
Identification and Allometric Scaling of Megamouth Shark (Megachasma pelagios) Mother-Offspring Specimens from Dipaculao, Aurora, Luzon Island, Philippines
The Catch and Trade of Seahorses in the Philippines Post-CITES (2019)

Journal Issue Volume 32 Issue 1 Dietary Vitamin C and Lipid Feed Supplementations Influence...

Research Article

Dietary Vitamin C and Lipid Feed Supplementations Influence the Expression of Immune-Related Genes in Litopenaeus vannamei (Boone 1931) Subjected to Thermal Stress

Eljenmer L. Sindo1,2 ORCID logo , Rex Ferdinand M. Traifalgar 2 ORCID logo

1 Institue of Fish Processing Technology, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo, Philippines
2 Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao 5023, Iloilo, Philippines
3 Institute of Bioinformatics and Systems Biology, College of Engineering Bioscience, National Yang Ming Chiao Tung University, Hsinchu, Taiwan

Page 43-55 | Received : May 28, 2023, Accepted : December 11, 2024

Abstract

This study investigated the effects of dietary vitamin C (vitC) and lipid on the expression of immune-related genes in Litopenaeus vannamei postlarvae subjected to thermal stress, with water temperature fluctuating from 27.0 ± 1.1 to 34.0 ± 1.8 °C for 15 days. Three experimental diets were formulated containing two different levels of vitC (1 and 3 g kg -1) and lipid (17 and 34 g kg -1). Significantly higher total hemocyte count (9.63 × 105 ± 3.28 × 105 cells ml -1) was observed in shrimp fed 3 g kg -1 vitC and 34 g kg -1 lipid diets. Using real-time quantitative polymerase chain reaction and the comparative 2-ΔΔCt method, the dietary vitC level of 3 g kg -1 significantly upregulated heat shock protein 90 (HSP90), heat shock protein 70 (HSP70), anti-lipopolysaccharide factor (ALF), crustin, and the Prophenoloxidase (proPO) system. Meanwhile, the dietary lipid level of 34 g kg -1 resulted in a significant upregulation of HSP90 and penaeidin 2 (PEN 2) genes. Regarding the growth and survival indices of the shrimps fed the formulated diets, no significant differences (P > 0.05) were observed. In conclusion, increased dietary supplementation of vitC (3 g kg -1 diet) and lipid (34 g kg -1 diet) upregulated the immune-related genes, enhancing the immunity of shrimps during thermal stress.


Keywords: Pacific white shrimp, dietary supplementation, vitamin C, lipid, fluctuating temperature, immunity